of neopterin concentrations in urine can be performed by HPLC.
In serum, plasma, cerebrospinal fluid or other protein-containing human
body fluids, immunoassays like ELISA or RIA are preferable.
products of immune activation: Neopterin
D, et al. Institute of Medical Chemistry and Biochemistry, University of
Innsbruck, Innsbruck, Austria
Manual of Clinical Laboratory Immunology. 4th Edition (Rose RR,
deMacario EC, Fahey JL, Friedman H, Penn GM, editors), The American Society
for Microbiology, Washington DC, 1992, 251-255)
enzyme-linked immunosorbent assay for neopterin detection in blood
donations compared with RIA and HPLC.
P, et al. Central Inst. Blood Transfusion and Immunology, University
Hospital, Innsbruck, Austria
Chem 1994; 40: 265-266)
of a new simple and rapid enzyme-linked immunosorbent assay kit for
J, et al. Immuno-Biological
Laboratories GmbH, Hamburg, Germany
Chem Lab Med 2000; 38: 345-353.)
A new commercially available enzyme-linked immunosorbent assay (ELISA) kit
has been evaluated for the measurement of neopterin concentrations in
serum, plasma and urine. This competitive ELISA is technically simple,
requires only small sample volume and is rapid to perform. The assay
procedure consists of sequential 1.5 h and 10 min room temperature
incubation steps. The ELISA is accurate, sensitive, specific, and precise.
Linear regression analysis of neopterin concentrations measured with the
new ELISA and with an established method yielded a highly significant
correlation (r = 0.99). The new assay is applicable to ELISA workstations,
thus enabling determination of neopterin in large series of samples. The
neopterin ELISA kit has been used in routine laboratory testing of blood
donations in a blood bank.